At present, the most convenient, easiest and easiest to achieve plant disease control effect is mainly the use of chemical pesticides. However, the large-scale use of chemical pesticides has caused serious harm to the environment and humans; biological control is less toxic to humans and animals, and it has gradually become the focus of people's research, and biological control has also become an important development trend. In order to study the optimal fermentation conditions of the Trichoderma E11 strain, this article takes the Trichoderma E11 strain as the test strain and uses the mycelium growth index as the index to determine the liquid fermentation broth, carbon source, nitrogen source, pH value, inoculation through relevant experiments The best combination of quantity, fermentation time and fermentation temperature. The research in this paper shows that the dry weight of mycelium in D-fructose culture solution is the largest at 4.0 (mg / ml); the mycelium yield of ammonium dihydrogen phosphate is 0.8 (mg / ml) and the dry weight of the mycelium is the largest; pH value is 6 The dry weight of the mycelium is the largest at 5.2 (mg / ml); the inoculation volume of 1.2mL (1.2%) or 1.7mL (1.7%) is the most suitable for mycelial growth; when the temperature reaches 25 ° C, the mycelium yield reaches the maximum The value is 556mg / 50nd. Therefore, with D-fructose as the carbon source, ammonium dihydrogen phosphate as the nitrogen source, the fermentation temperature is 25 ℃, the initial pH of the medium is 6, and the inoculation volume is 1.2mL or 1.7ml.

Tao Liu. Screening of Liquid Fermentation Conditions of Biocontrol Fungus Trichoderma E11 Strain. Academic Journal of Agricultural Sciences (2021), Vol. 2, Issue 1: 24-37. https://doi.org/10.38007/AJAS.2021.020103.

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