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International Journal of Public Health and Preventive Medicine, 2020, 1(3); doi: 10.38007/IJPHPM.2020.010304.

Changes of Free Amino Acids in Skeletal Muscle and Serum after Exercise Injury

Author(s)

Xue Li

Corresponding Author:
Xue Li
Affiliation(s)

School of Design, Zhejiang Tech University, Hangzhou, Zhejiang 315100, China

Abstract

The theme of this paper is the change of free amino acid content in skeletal muscle and serum after sports injury. We selected 28 healthy athletes by exclusion and inclusion criteria. They were randomly divided into two groups. The intervention group consisted of 3 h group, 6 h group, 12 h group, 1 D group, 3 D group, 1 W Group and 3 W Group, respectively. After 3 hours, 6 hours, 12 hours, 1 day, 3 days, 1 week and 3 weeks after exercise, tissue sections were established abdominal venous blood was centrifuged to obtain serum. Some skin tissues of skeletal muscle of athletes were extracted and stained with black gold and he, and then the content of free amino acids in skeletal muscle and serum was detected. GraphPad prism 9.0 was used for statistical analysis. According to the experimental results, we found that most of the free amino acids in serum showed a downward trend within 3 hours, and the contents of aspartic acid and glutamine reached the maximum value on the third day. The contents of glycine, threonine, arginine and proline all reached the maximum after one week; in the distribution of free amino acids in skeletal muscle, the contents of proline, glycine and glutamine reached the maximum after days, while the contents of hydroxyproline, aspartic acid and serine reached the maximum within one day; the content of aspartic acid in serum was generally higher than that in skeletal muscle Glycine is an essential amino acid for human body. The content of free amino acids in serum was affected by bone metabolism. The content of amino acids in serum began to rise on the third day, and the content of free amino acids in bone gradually increased after three days. Once some amino acids decrease, it is difficult to recover quickly. It can be seen that timely supplement of amino acids can treat sports injury in time.

Keywords

Sports Injury, Skeletal Muscle, Free Amino Acids, Black Gold Ⅱ Staining

Cite This Paper

Xue Li. Changes of Free Amino Acids in Skeletal Muscle and Serum after Exercise Injury. International Journal of Public Health and Preventive Medicine (2020), Vol. 1, Issue 3: 38-49. https://doi.org/10.38007/IJPHPM.2020.010304.

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