How to improve the bone healing speed and bone healing quality of large bone tissue is the current research hotspot. Capillary vascularization is the initial and most basic link, which has the decisive influence. If it is not possible to form an effective blood circulation, the manufacture of related bone tissue engineering materials can only be limited to a small range. Only by further exploring the status and method of action in the healing of bone tissue can we provide a useful method for solving their doubts. The purpose of this paper is to establish a research model for vascular nerve that can be used in bone tissue mechanisms, using composite mold technology to study vascularized bone tissue, observing the effects of sensory nerve bundles and motor nerve bundles on the growth and reconstruction of bone tissue. Whether the capillary bundle has a subtle influence on the development of bone tissue when the bone healed in bone tissue. To explore the role of vascular nerve in bone tissue construction, and to analyze the effects of different types of nerve fibers on bone healing. In this paper, by preparing vascular nerved bone tissue experiments, compared the influence of nerves and capillaries on bone healing in regenerated bone healing tissues from tissue immunology. And use immunological staining to initially detect the mechanism that promotes bone healing. Using the combination of mold technology and surgical methods, applying sensory nerves, motor nerves, and the combination of the two into the bone healing tissue, analyzed the influence and mechanism of nerves on bone healing in bone healing tissues. The results of the study show that bone tissue is originally accompanied by the growth of nerve fibers and the growth of nerve fibers is parallel to the process of bone healing. During the healing of bones in bone tissue, the sensory nerves or sensory nerves contact the motor nerves can promote bone healing. However, there is no obvious promotion effect when simply placed in the motor nerve, and the regenerated nerve fiber is very meaningful for the new capillaries in bone healing.

Marquez Diego. Experimental Examination of Vascularized Bone Tissue Based on Combined Mold Technology. International Journal of Public Health and Preventive Medicine (2022), Vol. 3, Issue 1: 1-13. https://doi.org/10.38007/IJPHPM.2022.030101.

[1] Dooley, J., Robacki, J., Jenkins, S., Wrisley, R., & Lee, P. C. (2016)“Development of Microlayer Blown Film Technology by Combining Film Die and Layer Multiplication Concepts”, Polymer Engineering & Science, 56(5), pp.598-604.

[2] Wang, X., Jin, J., & Lei, D. (2017)“ Review:State-of-The-Art of Stamping-Forging Process With Sheet Metal Blank”, Journal of Harbin Institute of Technology, 24(3), pp.1-16.

[3] S.M, & Xiong. (2016)“Correlation Between Porosity and Fracture Mechanism in High Pressure Die Casting of Am60b Alloy”, Journal of Materials Science & Technology, 32(1), pp.54-61.

[4] Stürzer, D., & Arnold, A. (2015)“Spectral Analysis and Long-time Behaviour of A Fokker-Planck Equation With A Non-Local Perturbation”, Mathematics, 25(1), pp.53-89.

[5] Kohls, H., Anderson, M., Dickerhoff, J., Weisz, K., Córdova, A., & Berglund, P., et al. (2015)“Selective Access to All Four Diastereomers of A 1,3‐Amino Alcohol by Combination of Aketo Reductase‐ and An Amine Transaminase‐Catalysed Reaction.”, Advanced Synthesis & Catalysis,357(8), pp.1808-1814.

[6] Sherwood, P. W. (2015)“Neue Mehrbasische Säuren Verbreitern Die Rohstoffgrundlage Für Alkydharze”,  European Journal of Lipid Science & Technology, 63(11), pp.1049-1051.

[7] Meinert, D. I. H., Senger, D. I. T., Wiebking, D. I. N., & Diegelmann, D. I. C. (2015)“The Plug-In Hybrid Technology of The New Bmw x5 Edrive”,  Mtz Worldwide, 76(5), pp.4-9.

[8] Sherwood, P. W. (2015)“Neue Polyole Für Die Anstrichmittel‐Industrie”, European Journal of Lipid Science & Technology, 62(4), pp.335-337.

[9] Kaufmann, H. P., Lüßling, T., & Karabatur, A. (2015)“ Über Den Nachweis Von Rüböl in Olivenöl Und Die Quantitative Papier‐Chromatographie Des Rüböls”, European Journal of Lipid Science & Technology, 58(11), pp.985-991.

[10] Wang, M. O., Vorwald, C. E., Dreher, M. L., Mott, E. J., Cheng, M. H., & Cinar, A., et al. (2015)“Evaluating 3D-Printed Biomaterials As Scaffolds for Vascularized Bone Tissue Engineering”, Advanced Materials, 27(1), pp.138-44.

[11] Griffin, K. S., Davis, K. M., Mckinley, T. O., Anglen, J. O., Tien-Min G. Chu, & Boerckel, J. D., et al. (2015)“Evolution of Bone Grafting: Bone Grafts and Tissue Engineering Strategies for Vascularized Bone Regeneration”, Clinical Reviews in Bone & Mineral Metabolism, 13(4), pp. 232-244.

[12] Roux, B. M., Cheng, M. H., & Brey, E. M. (2015)“Engineering Clinically Relevant Volumes of Vascularized Bone”, Journal of Cellular & Molecular Medicine, 19(5), pp.903-914.

[13] Nakano, K., Murata, K., Omokawa, S., Akahane, M., Shimizu, T., & Kawamura, K., et al. (2016)“Promotion of Osteogenesis and Angiogenesis in Vascularized Tissue-Engineered Bone Using Osteogenic Matrix Cell Sheets”, Plastic & Reconstructive Surgery, 137(5), pp.1476-1484.

[14] Eweida, A., Fathi, I., Eltawila, A. M., Elsherif, A. M., Elkerm, Y., & Harhaus, L., et al. (2017)“Pattern of Bone Generation After Irradiation in Vascularized Tissue Engineered Constructs”, Journal of Reconstructive Microsurgery, 34(02), pp.130-137.

[15] Rao, R. R., Vigen, M. L., Peterson, A. W., Caldwell, D. J., Putnam, A. J., & Stegemann, J. P. (2015)“Dual-Phase Osteogenic and Vasculogenic Engineered Tissue for Bone Formation”, Tissue Eng Part A, 21(3-4), pp.530-540.

[16] Blache, U., Metzger, S., Vallmajomartin, Q., Martin, I., Djonov, V., & Ehrbar, M. (2016)“Dual Role of Mesenchymal Stem Cells Allows for Microvascularized Bone Tissue-Like Environments in Peg Hydrogels”,  Advanced Healthcare Materials, 5(4), pp.489-498.

[17] Ma, D., Ren, L., Cao, Z., Li, J., Cao, J., & Tian, W., et al. (2016)“Prefabrication of Axially Vascularized Bone By Combining β-Tricalciumphosphate, Arteriovenous Loop, and Cell Sheet Technique”, Tissue Engineering & Regenerative Medicine, 13(5), pp.579-584.

[18] Liu, Y., Chan, J. K., & Teoh, S. H. (2015)“Review of Vascularised Bone Tissue-Engineering Strategies With A Focus on Co-Culture Systems”, Journal of Tissue Engineering & Regenerative Medicine, 9(2), pp.85-105.

[19] Patrick, N., & Payatakes, A. (2017)“Vascularized Spare Parts Reconstruction of Hand Gunshot Injury”, The Journal of Hand Surgery (Asian-Pacific Volume), 22(03), pp.391-395.

[20] Eweida, A., Fathi, I., Eltawila, A. M., Elsherif, A. M., Elkerm, Y., & Harhaus, L., et al. (2017)“Pattern of Bone Generation After Irradiation in Vascularized Tissue Engineered Constructs”, Journal of Reconstructive Microsurgery, 34(02), pp.130-137.