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Zoology and Animal Physiology, 2021, 2(2); doi: 10.38007/ZAP.2021.020203.

Transcription and Translation Levels of PGC-1α in Skeletal Muscle of C57BL6 Mice


Kazuyoshier Yoshii

Corresponding Author:
Kazuyoshier Yoshii

University of Sydney, Australia


Skeletal muscle plays a very important role in the growth and development of the human body, but with the increase of age, the activity of various substances in skeletal muscle will also be limited. This study mainly explored the transcription and translation levels of PGC-1α in skeletal muscle of C57BL6 mice under aerobic exercise. Select 60 C57BL6 mice (N=4), the training time of the mice is fixed at 10:00, use zH-PT to control the animal's running path, and monitor the entire exercise process to confirm the exercise intensity of the mice. After the 9th week of training, the mouse was hunger striked for 6 hours, and his neck was broken and killed. The thigh bones, calf bones and thigh muscles closest to the surface of the thigh bones were obtained. The OD value was measured with a spectrophotometer, and a reverse transcription system was prepared based on an efficient cDNA inversion system. The reverse recording reaction was performed in the MyCyclePCR amplification device. After high-fat diet, the discovery of PGC-1α in skeletal muscle of mice was significantly higher than that of normal group, that is, the discovery of PGC-1α in HC group was 36.5% higher than that of NC group (P<0.05). The level of PGC-1α in skeletal muscle of HE group was 13% lower than that of HC group (P<0.05). The results show that high-fat diet can greatly increase the performance level of skeletal muscle PGC-1α, while aerobic exercise can greatly reduce the performance level of skeletal muscle PGC-1α. Appropriate aerobic exercise can reduce PGC-1α transcription and translation levels in skeletal muscle of C57BL6 mice.


PGC-1α Transcription, Skeletal Muscle, C57BL6 Mice, Aerobic Exercise, Protein Levels

Cite This Paper

Kazuyoshier Yoshii. Transcription and Translation Levels of PGC-1α in Skeletal Muscle of C57BL6 Mice. Zoology and Animal Physiology (2021), Vol. 2, Issue 2: 27-40. https://doi.org/10.38007/ZAP.2021.020203.


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