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International Journal of World Medicine, 2024, 5(1); doi: 10.38007/IJWM.2024.050110.

Changes in the Expression Level of S100A8/A9 in Brain Tissue and Serum of Sleep-Deprived Mice


Yige Zheng, Qian Zhai, Wei Gao

Corresponding Author:
Wei Gao

Department of Anesthesiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, Shaanxi 710061, China


To explore the expression of S100A8/A9 in the brain and serum of chronic sleep deprivation (CSR) mice. The protein expression of S100A8/A9 in the hippocampus of CSR mice and wild-type mice was detected by western blotting, the mRNA expression of S100A8/A9 in the hippocampus of CSR mice and wild-type mice was detected by RNA-sequencing, and the serum concentration of S100A8/A9 in CSR mice and wild type mice was detected by enzyme-linked immunosorbent assay (ELISA). 1) The protein expression of S100A8/A9 in the hippocampus of the CSR group was significantly lower than that in wild type group (P<0.05); 2) The mRNA expression of S100A8/A9 in the hippocampus of the CSR group was significantly lower than that in wild type group(P<0.05) 3) The serum concentration of S100A9 of CSR mice was significantly higher than that in wild type group (P < 0.0001), but there was no significant difference in the S100A8 expression between the CSR group and the control group. The expression of S100A8/A9 in the brain and serum of CSR mice is different, and we need more studies to explore the internal mechanism.


S100A8/A9, Sleep Deprivation, Sleep Loss

Cite This Paper

Yige Zheng, Qian Zhai, Wei Gao. Changes in the Expression Level of S100A8/A9 in Brain Tissue and Serum of Sleep-Deprived Mice. International Journal of World Medicine (2024), Vol. 5, Issue 1: 87-93. https://doi.org/10.38007/IJWM.2024.050110.


[1] Zhou M, Wang H, Zhu J, Chen W, Wang L, Liu S, Li Y, Wang L, Liu Y, Yin P, Liu J, Yu S, Tan F, Barber RM, Coates MM, Dicker D, Fraser M, González-Medina D, Hamavid H, Hao Y, Hu G, Jiang G, Kan H, Lopez AD, Phillips MR, She J, Vos T, Wan X, Xu G, Yan LL, Yu C, Zhao Y, Zheng Y, Zou X, Naghavi M, Wang Y, Murray CJ, Yang G, Liang X. Cause-specific mortality for 240 causes in China during 1990-2013: a systematic subnational analysis for the Global Burden of Disease Study 2013. Lancet. 2016 Jan 16;387(10015):251-72.

[2] Bandyopadhyay A, Sigua NL. What Is Sleep Deprivation? Am J Respir Crit Care Med. 2019 Mar 15;199(6):P11-P12. 

[3] van Leeuwen WM, Lehto M, Karisola P, Lindholm H, Luukkonen R, Sallinen M, Härmä M, Porkka-Heiskanen T, Alenius H. Sleep restriction increases the risk of developing cardiovascular diseases by augmenting proinflammatory responses through IL-17 and CRP. PLoS One. 2009;4(2):e4589. 

[4] Chennaoui M, Gomez-Merino D, Drogou C, Geoffroy H, Dispersyn G, Langrume C, Ciret S, Gallopin T, Sauvet F. Effects of exercise on brain and peripheral inflammatory biomarkers induced by total sleep deprivation in rats. J Inflamm (Lond). 2015 Sep 30;12:56. 

[5] Bellesi M, de Vivo L, Chini M, Gilli F, Tononi G, Cirelli C. Sleep Loss Promotes Astrocytic Phagocytosis and Microglial Activation in Mouse Cerebral Cortex. J Neurosci. 2017 May 24;37(21):5263-5273. 

[6] Kessel C, Lavric M, Weinhage T, Brueckner M, de Roock S, Däbritz J, Weber J, Vastert SJ, Foell D. Serum biomarkers confirming stable remission in inflammatory bowel disease. Sci Rep. 2021 Mar 23;11(1):6690.

[7] Soyfoo MS, Roth J, Vogl T, Pochet R, Decaux G. Phagocyte-specific S100A8/A9 protein levels during disease exacerbations and infections in systemic lupus erythematosus. J Rheumatol. 2009 Oct;36(10):2190-4. 

[8] Kang KY, Woo JW, Park SH. S100A8/A9 as a biomarker for synovial inflammation and joint damage in patients with rheumatoid arthritis. Korean J Intern Med. 2014 Jan;29(1):12-9. 

[9] Guo D, Zhu Z, Xu T, Zhong C, Wang A, Xie X, Peng Y, Peng H, Li Q, Ju Z, Geng D, Chen J, Liu L, Wang Y, Zhang Y, He J. Plasma S100A8/A9 Concentrations and Clinical Outcomes of Ischemic Stroke in 2 Independent Multicenter Cohorts. Clin Chem. 2020 May 1;66(5):706-717.

[10] Hagmeyer S, Romão MA, Cristóvão JS, Vilella A, Zoli M, Gomes CM, Grabrucker AM. Distribution and Relative Abundance of S100 Proteins in the Brain of the APP23 Alzheimer's Disease Model Mice. Front Neurosci. 2019 Jun 20;13:640. 

[11] Zhang, Shuyue., Zhang, Ying., Zheng, Yige., Zhu, Shan., Sun, Jianyu..  Dexmedetomidine attenuates sleep deprivation-induced inhibition of hippocampal neurogenesis via VEGF-VEGFR2 signaling and inhibits neuroinflammation. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023,.

[12] Isidor B, Poignant S, Corradini N, Fouassier M, Quartier P, Roth J, Picherot G. Hyperzincemia and hypercalprotectinemia: unsuccessful treatment with tacrolimus. Acta Paediatr. 2009 Feb;98(2):410-2. 

[13] Vogl T, Ludwig S, Goebeler M, Strey A, Thorey IS, Reichelt R, Foell D, Gerke V, Manitz MP, Nacken W, Werner S, Sorg C, Roth J. MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytes. Blood. 2004 Dec 15;104(13):4260-8.

[14] Han C, Huang H, Hu M, Wang Q, Gao Y, Liu Y. Time-dependent expression of leukotriene B4 receptors in rat collagen-induced arthritis. Prostaglandins Other Lipid Mediat. 2007 May;83(3):225-30. 

[15] McHugh J. A protective role for epidermal S100A9 in PsA. Nat Rev Rheumatol. 2022 Sep;18(9):495.

[16] Pruenster M, Kurz AR, Chung KJ, Cao-Ehlker X, Bieber S, Nussbaum CF, Bierschenk S, Eggersmann TK, Rohwedder I, Heinig K, Immler R, Moser M, Koedel U, Gran S, McEver RP, Vestweber D, Verschoor A, Leanderson T, Chavakis T, Roth J, Vogl T, Sperandio M. Extracellular MRP8/14 is a regulator of β2 integrin-dependent neutrophil slow rolling and adhesion. Nat Commun. 2015 Apr 20;6:6915. 

[17] Peng S, Sun X, Wang X, Wang H, Shan Z, Teng W, Li C. Myeloid related proteins are upregulated in autoimmune thyroid diseases and activate toll-like receptor 4 and pro-inflammatory cytokines in vitro. Int Immunopharmacol. 2018 Jun;59:217-226. 

[18] Wang S, Song R, Wang Z, Jing Z, Wang S, Ma J. S100A8/A9 in Inflammation. Front Immunol. 2018 Jun 11;9:1298.

[19] Goldberg EL, Asher JL, Molony RD, Shaw AC, Zeiss CJ, Wang C, Morozova-Roche LA, Herzog RI, Iwasaki A, Dixit VD. β-Hydroxybutyrate Deactivates Neutrophil NLRP3 Inflammasome to Relieve Gout Flares. Cell Rep. 2017 Feb 28;18(9):2077-2087.

[20] Abu El-Asrar AM, Alam K, Siddiquei MM, Van den Eynde K, Mohammad G, De Hertogh G, Opdenakker G. Myeloid-Related Protein-14/MRP-14/S100A9/Calgranulin B is Associated with Inflammation in Proliferative Diabetic Retinopathy. Ocul Immunol Inflamm. 2018;26(4):615-624. 

[21] Chimenti MS, Triggianese P, Botti E, Narcisi A, Conigliaro P, Giunta A, Teoli M, Perricone R, Costanzo A. S100A8/A9 in psoriatic plaques from patients with psoriatic arthritis. J Int Med Res. 2016 Sep;44(1 suppl):33-37.

[22] Xu Ping. Relationship between S100A8/A9 and systemic lupus erythematosus and renal damage. Tianjin: graduate School of Tianjin Medical University, 2017 Vol 43-35

[23] Van Lent PL, Grevers L, Blom AB, Sloetjes A, Mort JS, Vogl T, Nacken W, van den Berg WB, Roth J. Myeloid-related proteins S100A8/S100A9 regulate joint inflammation and cartilage destruction during antigen-induced arthritis. Ann Rheum Dis. 2008 Dec;67(12):1750-8..

[24] Bouma G, Lam-Tse WK, Wierenga-Wolf AF, Drexhage HA, Versnel MA. Increased serum levels of MRP-8/14 in type 1 diabetes induce an increased expression of CD11b and an enhanced adhesion of circulating monocytes to fibronectin. Diabetes. 2004 Aug;53(8):1979-86. 

[25] Pruenster M, Vogl T, Roth J, Sperandio M. S100A8/A9: From basic science to clinical application. Pharmacol Ther. 2016 Nov;167:120-131.

[26] Guo Daoxia. Relationship between S100A8/A9 and Netrin-1 levels and prognosis of ischemic stroke. Suzhou University, 2020.

[27] Zhao Donglin, Xiong Chao, Li Dekui. Effect of drilling and drainage on serum S100A8/A9 level and brain edema in patients with intracerebral hemorrhage. Journal of Cardiovascular and Cerebrovascular Diseases of Integrated traditional Chinese and Western Medicine, 2017 15 (13): 1656-1658.

[28] Pu Zhidan. Study on the expression of S100A8/A9 in serum of patients with acute arteriosclerotic cerebral infarction. Guangzhou Medical University, 2017.