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International Journal of Health and Pharmaceutical Medicine, 2020, 1(2); doi: 10.38007/IJHPM.2020.010201.

Effect of Invigorating Spleen Herbs on Apoptosis of Transplanted Human Gastric Cancer Cells in Nude Mice


Qingqing Shen

Corresponding Author:
Qingqing Shen

Yunnan University of Traditional Chinese Medicine, Yunnan, China


Gastric cancer is the most common disease among various malignant tumors in China. Moreover, the incidence rate of gastric cancer is obviously different from that of gender and age. The incidence of gastric cancer in the northwest and east coastal areas is significantly higher than that in the south. The incidence of gastric cancer is 50 years old, and the ratio of male to female is about 2:1. However, with the development of economy, many people's diet structure has changed, and the work pressure is increasing, which leads to the younger gastric cancer. Therefore, the study of gastric cancer cells is very important. In view of the current social situation, this paper proposed the possible relationship between spleen strengthening Chinese medicine and gastric cancer cells, and studied the nude mice as the experimental model of the experiment. In this paper, the spleen invigorating traditional Chinese medicine compound coix seed, Atractylodes macrocephala and Jianpi pill were used as the basis of spleen strengthening drugs. In this study, nude mice with human gastric cancer cells were used as the model, and the subcutaneous tissue of nude mice was injected into the tumor model. The apoptosis of human gastric cancer cells in nude mice was observed. The antitumor effect, apoptosis rate and morphological changes of tumor cells were observed by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). The content of VEGF protein was detected by ELISA, and the activities of Mg2 + - ATPase and G-6-Pase in lateral tissues were detected by chromogenic method. The results showed that the number of gastric cancer cells in nude mice induced by Jianpi traditional Chinese medicine was much less than that in nude mice without spleen invigorating Chinese medicine, which indicated that spleen strengthening Chinese medicine had a good inhibitory effect on gastric cancer cells, and inducing apoptosis of gastric cancer cells might be one of the mechanisms of anti-cancer effect of spleen strengthening Chinese medicine.


Spleen Invigorating Herbs, Gastric Cancer Cells, Carcinogenesis Model, Apoptosis of Gastric Cancer Cells

Cite This Paper

Qingqing Shen. Effect of Invigorating Spleen Herbs on Apoptosis of Transplanted Human Gastric Cancer Cells in Nude Mice. International Journal of Health and Pharmaceutical Medicine (2020), Vol. 1, Issue 2: 1-12. https://doi.org/10.38007/IJHPM.2020.010201.


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