Welcome to Scholar Publishing Group

International Journal of Health and Pharmaceutical Medicine, 2021, 2(1); doi: 10.38007/IJHPM.2021.020104.

Clinical Application of Confocal Microscope in Diagnosis and Treatment of Fungal Keratitis


Deifei Sun

Corresponding Author:
Deifei Sun

Inner Mongolia University of Science & Technology, Inner Mongolia, China


To explore the clinical application of confocal microscopy combined with corneal scraping in fungal keratitis. In this paper, 50 patients with clinical diagnosis of fungal keratitis were examined by hybrid microscope and keratotomy, postoperative microscope and fungal culture, and drug or combined surgical treatment, and drug or combined surgical treatment was performed, and the positive rate of diagnosis was counted. The positive rate of confocal microscopy diagnosis at admission was 70%, and the positive rate of corneal scraping pathogen diagnosis was 80%. It is mainly because they have received different degrees of treatment before this, which has an impact on this initial clinical diagnosis. After treatment, 35 eyes (79%) received clinical care through simple medication, while 15 eyes (21%) required surgery to treat infections that could not be controlled by simple medication. Confocal microscopy has the characteristics of non-invasive, rapid, and good repeatability. Combined with laboratory examination of corneal scraping can improve the diagnostic positive rate of fungal keratitis and better guide the treatment.


Confocal Microscopy, Fungal Keratitis, Keratitis Diagnosis, Clinical Application

Cite This Paper

Deifei Sun. Clinical Application of Confocal Microscope in Diagnosis and Treatment of Fungal Keratitis. International Journal of Health and Pharmaceutical Medicine (2021), Vol. 2, Issue 1: 39-48. https://doi.org/10.38007/IJHPM.2021.020104.


[1] Wu, X, Tao, Y., Qiu, Q., & Wu, X. (2018). “Application of Image Recognition-Based Automatic Hyphae Detection in Fungal Keratitis”, Australasian Physical & Engineering Ences in Medicine, 41(1), 95-103.

[2] Jaya Devi Chidambaram, Namperumalsamy Venkatesh Prajna, Natasha Larke, David Macleod, Palepu Srikanthi, & Shruti Lanjewar. (2017). “In Vivo Confocal Microscopy Appearance of Fusarium and Aspergillus Species in Fungal Keratitis”, Br J Ophthalmol, 101(8), 1119-1123. DOI: 10.1136/bjophthalmol-2016-309656

[3] Cuiying Zhang, Shufang Wei, Liying Zhang, Weijing Li, Yan Lv, & Guoying Mu. (2016). “Observation of Curative Effect Regarding Corneal Cross-Linking Treatment of Riboflavin Combined With 440 Nm Blue-Light Cornea for Fungal Keratitis”, International Journal of Clinical & Experimental Medicine, 9(2), 717-724.

[4] Daas L, Viestenz A, Bischoff M, Hasenfus A, & Seitz B. (2016). “Confocal Microscopy for the Diagnostics of Fungal Keratitis”, Ophthalmologe, 113(9), 767-771. DOI: 10.1007/s00347- 015-0206-4

[5] Padzik, M., Szaflik, J. P., Baltaza, W., Perkowski, K., & Chomicz, L. (2017). “In Vivo Confocal Microscopy and in Vitro Culture Techniques as Tools for Evaluation of Severe Acanthamoeba Keratitis Incidents”, Ann Parasitol, 63(4), 341-346. DOI: 10.17420/ap6304.121

[6] Füst, ágnes, Tóth, Jeannette, Simon, G., Imre, László, & Nagy, Zoltán Z. (2017). “Specificity of in Vivo Confocal Cornea Microscopy in Acanthamoeba Keratitis”, European Journal of Ophthalmology, 27(1), 10-15. DOI: 10.5301/ejo.5000817

[7] Stephen? Tak-Lun, L., Evan?Po-Fat, Y., Angela? Hiu-Yan, W., John? Chun-Ting, Y., & Lester? Wang-Hon, Y. (2016). “Successful Treatment of Lasiodiplodia Theobromae Keratitis – Assessing the Role of Voriconazole”, Case Reports in Ophthalmology, 7(3), 179-185. DOI: 10.1159/000449369

[8] Szentmáry N, Módis L, Imre L, Füst Á, Daas L, & Laurik L. (2017). “Diagnostics and Treatment of Infectious Keratitis”, orvosi hetilap, 158(31), pp.1203.

[9] Perrot JL, Julienne R, Kaspi M, Labeille B, Grivet D, & Vercherin A. (2016). “The Role of Reflectance Confocal Microscopy in the Diagnosis of Ocular-Cutaneous Erucism or Dermatitis and Keratitis Induced by Pine Processionary Caterpillar Hairs”, annales de dermatologie et de venereologie, 143(12), 860. DOI: 10.1016/j.annder.2016.07.013

[10] Marchenko, N. R., & Kasparova, E. A. (2016). “Diagnosis of Acanthamoeba Keratitis”, Vestnik oftalmologii, 132(5), 103. DOI: 10.17116/oftalma20161325103-109

[11] Qu, J. H., Wang, Z. Q., Zhang, Y., & Sun, X. G. (2017). “Clinical Study on the Corneal Epithelial Dysfunction after Cataract Extraction”, Zhonghua yan ke za zhi Chinese Journal of Ophthalmology, 53(3), 188. DOI: 10.3760/cma.j.issn.0412-4081.2017.03.008

[12] Ramappa, M., Nagpal, R., Sharma, S., & Chaurasia, S. (2017). “Successful Medical Management of Presumptive Pythium Insidiosum Keratitis”, Cornea, 36(4), 511-514.