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International Journal of Health and Pharmaceutical Medicine, 2022, 3(2); doi: 10.38007/IJHPM.2022.030205.

Interference with TRIM26 Expression on Proliferation of Glioma Cells


Rashar Almajed

Corresponding Author:
Rashar Almajed

American University in the Emirates, UAE


Colloid tumor, as the most common malignant tumor in the brain, seriously endangers human life and health. TRIM 26 expression is closely related to the rapid growth and cell metastasis of most cancer cells. This article mainly studies the effect of interference with TRIM 26 expression on the proliferation of colloid tumor cells. Randomly selected 60 cases of colloid tumor specimens and the brain tissue of 10 cases of brain trauma patients as a normal control group. First, cell culture is performed to observe the growth status of the cells. Subsequently, the SiRNA interference technique was used to interfere with the expression of TRIM 26 on the liver cancer cell lines HepG 2 and Bel-720. The cells were collected for TRIM 26 protein westernBlot and fluorescence quantitative PCR to verify the knockout effect, and the cell proliferation, clone formation, transfer, and invasion experiments were performed. Experimental data showed that among 60 glioma patients, TRIM26 protein samples were positive in 46 cases, and 10 normal brain tissue samples were all negative.The performance of TRIM26 glioma was compared with the performance in normal brain tissue. The difference was significant and statistically significant (p<0.01). The results show that TRIM 26 can reduce the protein level of PBX1 through the correlation of time gradient and concentration gradient. TRIM 26 can reduce the mRNA level of RNF6, the downstream target gene of PBX1, without affecting the mRNA level of PBX1.


TRIM26, Glioma Cells, Cell Proliferation, Cell Migration

Cite This Paper

Rashar Almajed. Interference with TRIM26 Expression on Proliferation of Glioma Cells. International Journal of Health and Pharmaceutical Medicine (2022), Vol. 3, Issue 2: 47-58. https://doi.org/10.38007/IJHPM.2022.030205.


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