The development and key technology research of zebrafish online biomonitoring technology can realize real-time monitoring of water quality safety from the perspective of ecosystem by means of 3s technology, and realize early warning of water quality according to the response characteristics of zebrafish. The purpose of this paper is to study the water quality monitoring and early warning technology of zebrafish behavior based on 3s technology. Use GIS technology to process a large number of graphics, data and models, combine graphics and mathematical models, and implement the results of operations on the map. By analyzing the factors that affect the behavior of the indicator biological zebrafish, the results show that these potential physical stress factors have an impact on the normal behavior of zebrafish, but they are also acceptable in the normal range. Zebrafish can adjust and adapt within the fluctuation range of normal water quality, such as pH value and dissolved oxygen, and the behavioral difference is not obvious.

Fawanz Almunlihi. Water Quality Monitoring and Early Warning Technology of Zebrafish Behavior Based on 3s Technology. Academic Journal of Environmental Biology (2021), Vol. 2, Issue 3: 39-46. https://doi.org/10.38007/AJEB.2021.020305.

[1] Meshalkina, Darya, A, et al. The Effects of Chronic Amitriptyline on Zebrafish Behavior and Monoamine Neurochemistry. Neurochemical Research, 2018, 43(6):1191-1199.

[2] Tolbert, Tyrone, J, et al. Tracking Nemo: Help Scientists Understand Zebrafish Behavior. Zebrafish, 2018, 15(3):310-313. https://doi.org/10.1089/zeb.2017.1542

[3] Pullaguri N, Nema S, Bhargava Y, et al. Triclosan alters adult zebrafish behavior and targets acetylcholinesterase activity and expression. Environmental toxicology and pharmacology, 2020, 75(Apr.):103311.1-103311.7. https://doi.org/10.1016/j.etap.2019.103311

[4] Pietro, De, Lellis, et al. Model-Based Feedback Control of Live Zebrafish Behavior via Interaction With a Robotic Replica. IEEE Transactions on Robotics, 2019, 36(1):28-41.

[5] Privat M, Sumbre G . Naturalistic Behavior: The Zebrafish Larva Strikes Back. Current Biology, 2020, 30(1):R27-R29. https://doi.org/10.1016/j.cub.2019.11.014

[6] Himanshu G, Srinithi P, Narasimha P, et al. Sodium benzoate induced developmental defects, oxidative stress and anxiety-like behaviour in zebrafish larva. Biochemical and Biophysical Research Communications, 2018, 502(3):364-369.

[7] Silva F, Rocha W, Santos S, et al. Acute hyperglycemia does not alter the exploratory behavior of adult zebrafish (Danio rerio). Journal of Experimental and Applied Animal Sciences, 2019, 3(1):1-6. https://doi.org/10.20454/jeaas.2019.1528

[8] De P, Orger M B . Social Behavior: A Neural Circuit for Social Behavior in Zebrafish. Current Biology, 2018, 28(15):R828-R830.

[9] Thompson W A, Vijayan M M . Zygotic Venlafaxine Exposure Impacts Behavioral Programming by Disrupting Brain Serotonin in Zebrafish. Environmental Science and Technology, 2020, 54(22):14578-14588. https://doi.org/10.1021/acs.est.0c06032

[10] Pullaguri N, Nema S, Bhargava Y, et al. Triclosan alters adult zebrafish behavior and targets acetylcholinesterase activity and expression. Environmental toxicology and pharmacology, 2020, 75(Apr.):103311.1-103311.7.

[11] Privat M, Sumbre G . Naturalistic Behavior: The Zebrafish Larva Strikes Back. Current Biology, 2020, 30(1):R27-R29. https://doi.org/10.1016/j.cub.2019.11.014

[12] Abreu M, Kalueff A V . Of mice and zebrafish: the impact of the experimenter identity on animal behavior. Lab Animal, 2021, 50(1):7-7.

[13] Pilehvar A, Town R M, Blust R . The effect of copper on behaviour, memory, and associative learning ability of zebrafish (Danio rerio). Ecotoxicology and Environmental Safety, 2020, 188(Jan.):109900.1-109900.10.

[14] Smith A, Kasper S . The Effects of Lavender Essential Oil on Anxious Behavior and Cortisol Levels in Zebrafish. The FASEB Journal, 2020, 34(S1):1-1.

[15] Ranjan S, Sharma P K . Study of Stress, Anxiety and Depression in Type 2 Diabetic Model of Zebrafish (Danio rerio). Bulletin of Pure & Applied Sciences- Zoology, 2020, 39A(1):25-35.

[16] Corradi L, Bruzzone M, MD Maschio, et al. Hypothalamic Galanin-producing neurons regulate stress in zebrafish through a peptidergic, self-inhibitory loop. Current Biology, 2021, 32(7):1497-1510.e5.

[17] Ishani M, Anuradha B . Temporal Patterns in Interactions Across Same- and Mixed-Sex Wild Zebrafish Dyads. Zebrafish, 2021, 18(5):307-315. https://doi.org/10.1089/zeb.2021.0012

[18] Bachour R L, Golovko O, Kellner M, et al. Behavioral effects of citalopram, tramadol, and binary mixture in zebrafish (Danio rerio) larvae. Chemosphere, 2020, 238(Jan.):124587.1-124587.7.

[19] Wicki A, Lehmann P, Hauck C, et al. Assessing the potential of soil moisture measurements for regional landslide early warning. Landslides, 2020, 17(8):1881-1896.

[20] Weigand M, Wagner F M, Limbrock J K, et al. A monitoring system for spatiotemporal electrical self-potential measurements in cryospheric environments. Geoscientific Instrumentation Methods and Data Systems, 2020, 9(2):317-336. https://doi.org/10.5194/gi-9-317-2020

[21] Khan R, Yousaf S, Haseeb A, et al. Exploring a Design of Landslide Monitoring System. Complexity, 2021, 2021(2):1-13. https://doi.org/10.1155/2021/5552417