International Journal of Health and Pharmaceutical Medicine, 2022, 3(4); doi: 10.38007/IJHPM.2022.030403.
University of Garden City, Sudan
With the progress and development of medical science, fluorescence microscope has been widely used in biological research. Muscle and tendon injuries, as common sports injuries, bring a lot of inconvenience to people's daily life and work. At present, the commonly used methods of repairing muscle and tendon injury have certain curative effect, but the treatment cycle is long and the recovery degree is low. In order to better detect the biological characteristics of cells, design muscle and tendon injury model, and explore the treatment of muscle and tendon injury, fluorescence microscope was introduced into the experimental process. Firstly, the muscle satellite cells and tendon stem cells of mice were cultured and passaged. After cryopreservation and resuscitation, the biological characteristics of the cultured cells were detected. The proliferation and cloning of the cells were observed by fluorescence microscope, and the growth curve was drawn. Then the muscle injury model and tendon injury model were established by cardiotoxin and collagenase type I respectively. Paraffin section was made for observation and serum CK index was detected. The experimental results show that fluorescence microscope can effectively observe the biological characteristics of cells and help to establish muscle tendon injury model. It is concluded that muscle satellite cells and tendon stem cells can recover the injury. This provides experimental basis for the clinical application of cell therapy in the treatment of muscle tendon injury.
Fluorescence Microscope, Muscle Injury, Tendon Injury, Animal Model, Biological Characteristics
Junmin Kimi. Cell Biological Characteristics by Fluorescence Microscope and Its Application in Animal Models of Muscle and Tendon Injuries. International Journal of Health and Pharmaceutical Medicine (2022), Vol. 3, Issue 4: 29-40. https://doi.org/10.38007/IJHPM.2022.030403.
 Putman, C. A. J. , Hansma, H. G. , Gaub, H. E. , & Hansma, P. K. . (2017). “Polymerized Lb Films Imaged with a Combined Atomic Force Microscope-Fluorescence Microscope”, Langmuir, 8(12), pp.3014-3019.https://doi.org/10.1021/la00048a027
 Zhi, J. J. , Yan, H. , & Sun, L. H. . (2018). “Establishment of an Acute Extraocular Muscle Injury Model in Cats”, International journal of ophthalmology, 11(09),pp.45-51.
 Suzuki, M. , Inage, K. , Sakuma, Y. , Orita, S. , Yamauchi, K. , & Suzuki, T. , et al. (2016). “Effect of Administration of Antibodies Against Nerve Growth Factor in a Rat Model of Muscle Injury”, Injury-international Journal of the Care of the Injured, 47(3), pp.609-612.https://doi.org/10.1016/j.injury.2015.11.026
 Gaut, C. , Sullivan, J. M. , Biscaro, B. , Soares, E. J. , Nicholson, K. , & Hoppin, J. , et al. (2020). “Spect Imaging of Muscle Injury with [99m tc] Mdp in a Mouse Model of Muscular Dystrophy”, Molecular Imaging and Biology, 22(3), pp.562-568.https://doi.org/10.1007/s11307-019-01394-7
 Freeberg, M. A. T. , Farhat, Y. M. , Easa, A. , Kallenbach, J. G. , Malcolm, D. W. , & Buckley, M. R. , et al. (2018). “Serpine1 Knockdown Enhances Mmp Activity After Flexor Tendon Injury in Mice: Implications for Adhesions Therapy”, Scientific Reports, 8(1), pp.5810.https://doi.org/10.1038/s41598-018-24144-1
 Wahl, D. J. , Jian, Y. , Bonora, S. , Zawadzki, R. J. , & Sarunic, M. V. . (2016). “Wavefront Sensorless Adaptive Optics Fluorescence Biomicroscope for in Vivo Retinal Imaging in Mice”, Biomedical Optics Express, 7(1), pp.1-12.https://doi.org/10.1364/BOE.7.000001
 Takao, H. , Suzuki, T. , Oana, H. , Washizu, M. , & Masuda, C. . (2016). “Characterisation of Optically Driven Microstructures for Manipulating Single DNA Molecules Under a Fluorescence Microscope”, Iet Nanobiotechnology, 10(3), pp.124.https://doi.org/10.1049/iet-nbt.2015.0036
 Elbahrawy, M. , Zakhem, E. , & Bitar, K. N. . (2016). “Tu1763 a Comparison Study of Muscle Progenitor Cells and Mesenchymal Stem Cells Injection in An Unrepaired Injury Model of External Anal Sphincter”, Gastroenterology, 150(4), pp.S937.https://doi.org/10.1016/S0016-5085(16)33174-2
 Kizilkaya, V. , Uruc, V. , Levent, A. , Kanat, O. , & Kalaci, A. . (2018). “Effectiveness of Ozone Therapy on Tendon Healing : An Experimental Study in Generated Achilles Tendon Injury Model in Rats”, Journal of Hard Tissue Biology, 27(4), pp.309-314.https://doi.org/10.2485/jhtb.27.309
 Zhang, J. , Yuan, T. , & Wang, H. C. . (2016). “Moderate Treadmill Running Exercise Prior to Tendon Injury Enhances Wound Healing in Aging Rats”, Oncotarget, 7(8), pp.8498-8512.https://doi.org/10.18632/oncotarget.7381
 Hajipour, B. , Navali, A. M. , Mohammad, S. A. , Mousavi, G. , Akbari, M. G. , & Miyandoab, T. M. , et al. (2016). “Phenytoin Accelerates Tendon Healing in a Rat Model of Achilles Tendon Rupture”, Bratisl Lek Listy,117(09), pp.543-546.https://doi.org/10.4149/BLL_2016_107
 Tucker, J. J. , Riggin, C. N. , Connizzo, B. K. , Mauck, R. L. , Steinberg, D. R. , & Kuntz, A. F. , et al. (2016). “Effect of Overuse-Induced Tendinopathy on Tendon Healing in a Rat Supraspinatus Repair Model”, Journal of Orthopaedic Research, 34(1), pp.161-166.https://doi.org/10.1002/jor.22993